WebAn E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermoph Properties & Usage Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer WebOct 31, 2014 · Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four …

BsaI Restriction Enzyme - Laboratory Notes

WebThermo Scientific Ppu21I (BsaAI) restriction enzyme recognizes YAC^GTR sites and cuts best at 30°C in its own unique buffer (isoschizomers: BsaAI, BstBAI). See Reaction … WebThis article contains a list of the most studied restriction enzymes whose names start with Bsa to Bso inclusive. It contains approximately 90 enzymes. The following information is … highbury ink london ontario

BsaI NEB

WebWe are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2024 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying … WebThermo Scientific Eco31I (BsaI) restriction enzyme recognizes GGTCTC (1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI). See Reaction … Web1 Supercoiled or high molecular weight DNA (e.g. plant genomic DNA) may require longer incubation time or higher amount of enzyme. 2 Some enzymes may require additional … how far is port stephens from sydney