Nephrin antibody

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August undefined, 2024

WebNephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the … WebAnti-NPHS1 nephrin antibodies are readily available from several suppliers. NPHS1 nephrin is a reported synonym of the human protein 'NPHS1 adhesion molecule, nephrin', encoded by the gene NPHS1. The full protein is reported to be 1241 amino acid residues in length. It is listed to be a member of the Immunoglobulin superfamily.

New Anti-Nephrin Antibody Mediated Podocyte Injury Model …

WebJan 10, 2024 · Next, the authors tried to determine if there were clinical differences between the clinical course of MCD with anti-nephrin antibodies and MCD without anti-nephrin … WebNephrin not only functions as the core component of the extracellular SD filtration network, ... 1193, and 1217 is the most widely characterized, potentially due to the commercial availability of phospho-specific antibodies for these sites . Phosphorylation at these sites results in the recruitment of Nck, PLC-γ1, ... patricia ocasio

Discovery of Autoantibodies Targeting Nephrin in Minimal

WebNephrin Antibodies. Antibodies that detect Nephrin can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Dot blot. These antibodies target Nephrin in Human, Mouse and Rat samples. Our Nephrin polyclonal and monoclonal antibodies are developed in Rabbit and Mouse. WebPurified by Protein A. Storage Buffer. Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Condition. Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Target. Gene ID. 4868. WebAnti-Nephrin (Nphs1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Nephrin is encoded by the Nphs1 gene in human. Nephrin is a structural protein making the slit diaphragm in the glomerular podocytes. patricia obituary nj

NEPHRIN antibody knockout validation Progen GP-N2

Phosphorylation of key podocyte proteins and the association with ...

WebNov 14, 2024 · Phosphorylation of nephrin can be induced using FKBP-FRB heterodimers. Nephrin is an essential structural component of the kidney glomerulus, a specialized structure for ultrafiltration of plasma, consisting of eight extracellular immunoglobulin G (IgG) repeats, a transmembrane domain, and an unstructured tail of ∼160 amino acids … Web353 NPHS1 Adhesion Molecule, Nephrin antibodies from 21 antibody suppliers Click each NPHS1 Adhesion Molecule, Nephrin antibody that interests you to view the full NPHS1 Adhesion Molecule, Nephrin antibody datasheet. View only NPHS1 Adhesion Molecule, Nephrin Monoclonal Antibodies or NPHS1 Adhesion Molecule, Nephrin … patricia ochiWebNephrin antibody was raised in guinea pig using a synthetic peptide corresponding to residues 1243-1256 of the intracellular domain of nephrin as the immunogen. Validations. None provided. Applications. Immunohistochemistry (IHC) Immunohistochemistry-paraffin (IHC-P) Western Blotting (WB) Reactivity. Homo sapiens (Human) Mus musculus … patricia oblinger atlanta mi

"WebAug 3, 2024 · nephrin Antibody (G-8) is a mouse monoclonal IgG 2a κ nephrin antibody, cited in 24 publications, provided at 200 µg/ml. raised against amino acids 23-322 mapping within an extracellular domain of nephrin of rat and human origin. nephrin Antibody (G-8) is recommended for detection of nephrin of mouse, rat and human origin by WB, IP, IF … " - Nephrin antibody

Nephrin antibody

WebMar 2, 2024 · The discovery of anti-nephrin antibody “dusting” in these biopsy specimens is accompanied by the presence of high levels of anti-nephrin antibody in the serum. It … WebNephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized …

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WebThus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3). Application Note. Anti-Nephrin Antibody has been tested for use in ELISA, Western Blotting, immunofluorescence, and immunohistochemistry. Expect a band at approximately 135 kDa in Western Blots of specific cell lysates and tissues. WebJul 6, 1999 · We describe here the size and location of nephrin, the first protein to be identified at the glomerular podocyte slit diaphragm. In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in …

WebNephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. WebELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB) Specificity. Recognizes nephrin, first described as a kidney podocyte marker protein (MW 135kD calculated from amino acid ...

WebSep 30, 2024 · We report our experience with a C57BL/6 FSGS model induced by polyclonal rabbit anti-mouse nephrin antibody (α-mNep Ab). Methods: α-mNep Ab, which was generated by genetic immunization, was ... WebAll lanes : Anti-Nephrin antibody [EPR20993] (ab216341) at 1/1000 dilution Lane 1 : Mouse kidney lysate Lane 2 : Mouse spleen lysate Lysates/proteins at 10 µg per lane. …

WebCells stained with primary antibody (green), and isotype control (orange). 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Seems to play a role in the development or function of the kidney glomerular filtration barrier.

WebThe antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit di… patricia o brien wraggehttp://www.nephjc.com/news/nephrin-mcd patricia ocelliWebNephrin Antibody detects endogenous levels of total Nephrin. RRID: AB_2844314 Cite Format: Affinity Biosciences Cat# AF7951, RRID:AB_2844314. Conjugate: Unconjugated. Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage: ... patricia ochmanWebJul 22, 2024 · nephrin Antibody (B-12): sc-377246 nephrin Antibody (B-12) is a mouse monoclonal IgG 2a κ, cited in 25 publications, provided at 200 µg/ml specific for an... patricia o carrollWebApr 14, 2024 · Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti Mouse Nephrin Antigen Affinity purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557 conjugated AntiGoat IgG Secondary Antibody (yellow; patricia ochalWebNephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, … patricia oconnorWebImmunohistochemical analysis of paraffin-embedded mouse kidney tissue using Nephrin Polyclonal Antibody (Product # PA5-20330) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody … patricia odegard